Under an initial investment scenario set at $ US2 M for the first year of implementation in the area of influence of the water fund, as well as for some of the micro-basins, the prioritization technique was applied through the RIOS program, routines that allowed the definition of priority sites within the area of influence according to predetermined watershed conservation objectives. To complete this prioritization process and generate detail in the investment portfolio, four specific objectives were identified: aquifer recharge, erosion control in springs, erosion control in surface waters, and flow regulation, and eight lines of control were established. strategic investment in order to promote and increase these objectives that come to be ecosystem services that will be generated from the interventions. The investment portfolio becomes a useful tool to demonstrate in a technical-scientific way where, how and when to invest.